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Hypochlorous acid

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Title: Hypochlorous acid  
Author: World Heritage Encyclopedia
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Subject: Hypochlorite, List of MeSH codes (D01), Chlorine, Hypobromous acid, Sodium hypochlorite
Collection: Disinfectants, Hydrogen Compounds, Hypochlorites, Mineral Acids, Oxidizing Acids, Oxidizing Agents
Publisher: World Heritage Encyclopedia

Hypochlorous acid

Hypochlorous acid
hypochlorous acid bonding
hypochlorous acid space filling
IUPAC name
hypochlorous acid, chloric(I) acid, chloranol, hydroxidochlorine
Other names
Hydrogen hypochlorite, Chlorine hydroxide
ChemSpider  Y
EC number 232-232-5
Jmol-3D images Image
Molar mass 52.46 g/mol
Appearance Colorless aqueous solution
Density Variable
Acidity (pKa) 7.53[1]
Main hazards Oxidizing Agent O
Related compounds
Related compounds
Calcium hypochlorite
Sodium hypochlorite
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
 Y  (: Y/N?)

Hypochlorous acid is a weak acid with the chemical formula HClO. It forms when chlorine dissolves in water, and it is HClO that actually does the disinfection when chlorine is used to disinfect water for human use. It cannot be isolated in pure form due to rapid equilibration with its precursor. HClO is an oxidizer, and as its sodium salt sodium hypochlorite, (NaClO), or its calcium salt calcium hypochlorite, (Ca(ClO)2) is used as a bleach, a deodorant, and a disinfectant.


  • Uses 1
  • Formation, stability and reactions 2
    • Chemical reactions 2.1
    • Reactivity of HClO with biomolecules 2.2
      • Reaction with protein sulfhydryl groups 2.2.1
      • Reaction with protein amino groups 2.2.2
      • Reaction with DNA and nucleotides 2.2.3
      • Reaction with lipids 2.2.4
  • Mode of disinfectant action 3
    • Inhibition of glucose oxidation 3.1
    • Depletion of adenine nucleotides 3.2
    • Inhibition of DNA replication 3.3
    • Protein unfolding and aggregation 3.4
  • Hypochlorites 4
    • Production of hypochlorites using electrolysis 4.1
  • Safety 5
  • Hypomide 6
    • Specifications 6.1
    • Uses 6.2
  • See also 7
  • References 8
  • External links 9


In alkenes to chlorohydrins.[2]

In biology, hypochlorous acid is generated in activated neutrophils by myeloperoxidase-mediated peroxidation of chloride ions, and contributes to the destruction of bacteria.[3][4][5]

In the cosmetics industry it is used as a skin cleansing agent, which benefits the body's skin rather than causing drying. It is also used in baby products, because baby skin is particularly sensitive and can be easily irritated.

In water treatment, hypochlorous acid is the active sanitizer in hypochlorite-based products (e.g. used in swimming pools).

In food service and water distribution, specialized equipment to generate weak solutions of HOCl from water and salt is sometimes used to generate adequate quantities of safe (unstable) disinfectant to treat food preparation surfaces and water supplies.[6][7]

Formation, stability and reactions

Addition of chlorine to water gives both hydrochloric acid (HCl) and hypochlorous acid (HClO):[8]

Cl2 + H2O HClO + HCl
Cl2 + 4 OH 2 ClO + 2 H2O + 2 e
Cl2 + 2 e 2 Cl

When acids are added to aqueous salts of hypochlorous acid (such as sodium hypochlorite in commercial bleach solution), the resultant reaction is driven to the left, and chlorine gas is formed. Thus, the formation of stable hypochlorite bleaches is facilitated by dissolving chlorine gas into basic water solutions, such as sodium hydroxide.

The acid can also be prepared by dissolving dichlorine monoxide in water; under standard aqueous conditions, anhydrous hypochlorous acid is impossible to prepare due to the readily reversible equilibrium between it and its anhydride:[9]

2 HOCl Cl2O + H2O K(0°C) = 3.55×103 dm3mol−1

The presence of light or transition metal oxides of copper, nickel, or cobalt accelerates the exothermic decomposition into hydrochloric acid and oxygen:[9]

2 Cl2 + 2 H2O → 4 HCl + O2

Chemical reactions

In aqueous solution, hypochlorous acid partially dissociates into the anion hypochlorite OCl:

HClO OCl + H+

Salts of hypochlorous acid are called hypochlorites. One of the best-known hypochlorites is NaClO, the active ingredient in bleach.

HClO is a stronger oxidant than chlorine under standard conditions.

2HClO(aq) + 2H+ + 2e Cl2(g) + 2H2O E = +1.63 V

HClO reacts with HCl to form chlorine gas:

HClO + HCl → H2O + Cl2

HClO reacts with alkanes to form chloroalkanes and water, illustrated here by its reaction with methane:

CH4 + HClO → CH3Cl + H2O

HClO reacts with water to form hydrochloric acid and hydrogen peroxide:

H2O + HClO → HCl + H2O2

HClO also reacts with organic alcohols to form chloroalkanes, producing hydrogen peroxide, shown by the reaction with methanol:

CH3OH + HClO → CH3Cl + H2O2

HClO reacts with amines to form chloramines and water. Reacting with ammonia:

NH3 + HClO → NH2Cl + H2O

HClO can also react with organic amines to replace the amino group with chloride, forming chloroalkanes and hydroxylamine. Reaction with methylamine:

CH3NH2 + HClO → CH3Cl + NH2OH

Reactivity of HClO with biomolecules

Hypochlorous acid reacts with a wide variety of biomolecules, including DNA, RNA,[5][10][11][12] fatty acid groups, cholesterol[13][14][15][16][17][18][19][20] and proteins.[8][16][21][22][23][24][25]

Reaction with protein sulfhydryl groups

Knox et al.[23] first noted that HClO is a sulfhydryl inhibitor that, in sufficient quantity, could completely inactivate proteins containing sulfhydryl groups. This is because HClO oxidises sulfhydryl groups, leading to the formation of disulfide bonds[26] that can result in crosslinking of proteins. The HClO mechanism of sulfhydryl oxidation is similar to that of chloramine, and may only be bacteriostatic, because, once the residual chlorine is dissipated, some sulfhydryl function can be restored.[22] One sulfhydryl-containing amino acid can scavenge up to four molecules of HOCl.[25] Consistent with this, it has been proposed that sulfhydryl groups of sulfur-containing amino acids can be oxidized a total of three times by three HClO molecules, with the fourth reacting with the α-amino group. The first reaction yields sulfenic acid (R-SOH) then sulfinic acid (R-SO2H) and finally R-SO3H. Sulfenic acids form disulfides with another protein sulfhydryl group, causing cross-linking and aggregation of proteins. Sulfinic acid and R-SO3H derivatives are produced only at high molar excesses of HClO, and disulfides are formed primarily at bacteriocidal levels.[12] Disulfide bonds can also be oxidized by HClO to sulfinic acid.[26] Because the oxidation of sulfhydryls and disulfides evolves hydrochloric acid,[12] this process results in the depletion HClO.

Reaction with protein amino groups

Hypochlorous acid reacts readily with amino acids that have 2NH groups promoted attack on the peptide bond, resulting in cleavage of the protein. McKenna and Davies[28] found that 10 mM or greater HClO is necessary to fragment proteins in vivo. Consistent with these results, it was later proposed that the chloramine undergoes a molecular rearrangement, releasing HCl and ammonia to form an amide.[29] The amide group can further react with another amino group to form a Schiff base, causing cross-linking and aggregation of proteins.[16]

Reaction with DNA and nucleotides

Hypochlorous acid reacts slowly with DNA and RNA as well as all nucleotides in vitro.[10][30] GMP is the most reactive because HClO reacts with both the heterocyclic NH group and the amino group. In similar manner, TMP with only a heterocyclic NH group that is reactive with HClO is the second-most reactive. AMP and CMP, which have only a slowly reactive amino group, are less reactive with HClO.[30] UMP has been reported to be reactive only at a very slow rate.[5][10] The heterocyclic NH groups are more reactive than amino groups, and their secondary chloramines are able to donate the chlorine.[12] These reactions likely interfere with DNA base pairing, and, consistent with this, Prütz[30] has reported a decrease in viscosity of DNA exposed to HClO similar to that seen with heat denaturation. The sugar moieties are nonreactive and the DNA backbone is not broken.[30] NADH can react with chlorinated TMP and UMP as well as HClO. This reaction can regenerate UMP and TMP and results in the 5-hydroxy derivative of NADH. The reaction with TMP or UMP is slowly reversible to regenerate HClO. A second slower reaction that results in cleavage of the pyridine ring occurs when excess HClO is present. NAD+ is inert to HClO.[12][30]

Reaction with lipids

Hypochlorous acid reacts with unsaturated bonds in lipids, but not saturated bonds, and the OCl ion does not participate in this reaction. This reaction occurs by hydrolysis with addition of chlorine to one of the carbons and a hydroxyl to the other. The resulting compound is a chlorhydrin.[13] The polar chlorine disrupts lipid bilayers and could increase permeability.[14] When chlorhydrin formation occurs in lipid bilayers of red blood cells, increased permeability occurs. Disruption could occur if enough chlorhydrin is formed.[13][19] The addition of preformed chlorhydrins to red blood cells can affect permeability as well.[15] Cholesterol chlorhydrins have also been observed,[14][17] but do not greatly affect permeability, and it is believed that Cl2 is responsible for this reaction.[17]

Mode of disinfectant action

Escherichia coli exposed to hypochlorous acid lose viability in less than 100 ms due to inactivation of many vital systems.[8][31][32][33][34] Hypochlorous acid has a reported LD50 of 0.0104–0.156 ppm[35] and 2.6 ppm caused 100% growth inhibition in 5 minutes.[28] However it should be noted that the concentration required for bactericidal activity is also highly dependent on bacterial concentration.[23]

Inhibition of glucose oxidation

In 1948, Knox et al.[23] proposed the idea that inhibition of glucose oxidation is a major factor in the bacteriocidal nature of chlorine solutions. He proposed that the active agent or agents diffuse across the cytoplasmic membrane to inactivate key sulfhydryl-containing enzymes in the glycolytic pathway. This group was also the first to note that chlorine solutions (HOCl) inhibit sulfhydryl enzymes. Later studies have shown that, at bacteriocidal levels, the cytosol components do not react with HOCl.[36] In agreement with this, McFeters and Camper[37] found that aldolase, an enzyme that Knox et al.[23] proposes would be inactivated, was unaffected by HOCl in vivo. It has been further shown that loss of sulfhydryls does not correlate with inactivation.[22] That leaves the question concerning what causes inhibition of glucose oxidation. The discovery that HOCl blocks induction of β-galactosidase by added lactose[38] led to a possible answer to this question. The uptake of radiolabeled substrates by both ATP hydrolysis and proton co-transport may be blocked by exposure to HOCl preceding loss of viability.[36] From this observation, it proposed that HOCl blocks uptake of nutrients by inactivating transport proteins.[21][36][37][39] The question of loss of glucose oxidation has been further explored in terms of loss of respiration. Venkobachar et al.[40] found that succinic dehydrogenase was inhibited in vitro by HOCl, which led to the investigation of the possibility that disruption of electron transport could be the cause of bacterial inactivation. Albrich et al.[5] subsequently found that HOCl destroys cytochromes and iron-sulfur clusters and observed that oxygen uptake is abolished by HOCl and adenine nucleotides are lost. It was also observed that irreversible oxidation of cytochromes paralleled the loss of respiratory activity. One way of addressing the loss of oxygen uptake was by studying the effects of HOCl on succinate-dependent electron transport.[41] Rosen et al.[34] found that levels of reductable cytochromes in HOCl-treated cells were normal, and these cells were unable to reduce them. Succinate dehydrogenase was also inhibited by HOCl, stopping the flow of electrons to oxygen. Later studies[32] revealed that Ubiquinol oxidase activity ceases first, and the still-active cytochromes reduce the remaining quinone. The cytochromes then pass the electrons to oxygen, which explains why the cytochromes cannot be reoxidized, as observed by Rosen et al.[34] However, this line of inquiry was ended when Albrich et al.[8] found that cellular inactivation precedes loss of respiration by using a flow mixing system that allowed evaluation of viability on much smaller time scales. This group found that cells capable of respiring could not divide after exposure to HOCl.

Depletion of adenine nucleotides

Having eliminated loss of respiration Albrich et al.[8] proposes that the cause of death may be due to metabolic dysfunction caused by depletion of adenine nucleotides. Barrette et al.[38] studied the loss of adenine nucleotides by studying the energy charge of HOCl-exposed cells and found that cells exposed to HOCl were unable to step up their energy charge after addition of nutrients. The conclusion was that exposed cells have lost the ability to regulate their adenylate pool, based on the fact that metabolite uptake was only 45% deficient after exposure to HOCl and the observation that HOCl causes intracellular ATP hydrolysis. It was also confirmed that, at bacteriocidal levels of HOCl, cytosolic components are unaffected. So it was proposed that modification of some membrane-bound protein results in extensive ATP hydrolysis, and this, coupled with the cells inability to remove AMP from the cytosol, depresses metabolic function. One protein involved in loss of ability to regenerate ATP has been found to be ATP synthetase.[21] Much of this research on respiration reconfirms the observation that relevant bacteriocidal reactions take place at the cell membrane.[21][38][42]

Inhibition of DNA replication

Recently it has been proposed that bacterial inactivation by HOCl is the result of inhibition of DNA replication. When bacteria are exposed to HOCl, there is a precipitous decline in DNA synthesis that precedes inhibition of protein synthesis, and closely parallels loss of viability.[28][43] During bacterial genome replication, the origin of replication (oriC in E. coli) binds to proteins that are associated with the cell membrane, and it was observed that HOCl treatment decreases the affinity of extracted membranes for oriC, and this decreased affinity also parallels loss of viability. A study by Rosen et al.[44] compared the rate of HOCl inhibition of DNA replication of plasmids with different replication origins and found that certain plasmids exhibited a delay in the inhibition of replication when compared to plasmids containing oriC. Rosen’s group proposed that inactivation of membrane proteins involved in DNA replication are the mechanism of action of HOCl.

Protein unfolding and aggregation

HOCl is known to cause post-translational modifications to proteins, the notable ones being cysteine and methionine oxidation. A recent examination of HOCl's bactericidal role revealed it to be a potent inducer of protein aggregation.[45] Hsp33, a chaperone known to be activated by oxidative heat stress, protects bacteria from the effects of HOCl by acting as a holdase, effectively preventing protein aggregation. Strains of E. coli and Vibrio cholerae lacking Hsp33 were rendered especially sensitive to HOCl. Hsp33 protected many essential proteins from aggregation and inactivation due to HOCl, which is a probable mediator of HOCl's bactericidal effects.


Hypochlorites are the salts of hypochlorous acid; commercially important hypochlorites are calcium hypochlorite and sodium hypochlorite.

Production of hypochlorites using electrolysis

Solutions of hypochlorites can be produced by electrolysis of an aqueous chloride solution. Chlorine gas is produced at the anode, while hydrogen forms at the cathode. Some of the chlorine gas produced will dissolve forming hypochlorite ions. Hypochlorites are also produced by the disproportionation of chlorine gas in alkaline solutions.


HOCl is a strong oxidizer and can form explosive mixtures.


Hypomide was a stable formulation of hypochlorous acid used as a hand and surface sanitizer in both the hospital and home environment. Before the advent of Hypomide, hypochlorous acid had limited use in the healthcare environment because its instability necessitated in-situ production by electrolysis involving special equipment. Nevertheless, its potency as an antimicrobial agent was recognised and hypochlorous acid is often relied upon by dentists, for example, to clean their tools and instruments. It is no longer commercially available.


Because hypochlorous acid kills gram positive and gram negative bacteria (even multi-resistant strains such as MRSA), fungi and viruses. A 7 log10 reduction equates to 99.99999% kill rate: 100 times greater than the required pass rate on the BS:EN 1276 standard test for sanitizers and disinfectants. Even against difficult-to-treat spores, such as the dangerous Clostridium difficile, Hypomide achieves a 5 log10 kill rate.[46]


Hypochlorous acid is non-toxic and is not an irritant.[47][48][49]

See also


  1. ^ Harris, Daniel C. (2009). "Exploring Chemical Analysis, Fourth Edition". p. 538. 
  2. ^ Unangst, P. C. "Hypochlorous Acid" in Encyclopedia of Reagents for Organic Synthesis (Ed: L. Paquette) 2004, J. Wiley & Sons, New York. doi:10.1002/047084289
  3. ^ Harrison, J. E., and J. Schultz (1976). "Studies on the chlorinating activity of myeloperoxidase". Journal of Biological Chemistry 251 (5): 1371–1374.  
  4. ^ a b c Thomas, E. L. (1979). "Escherichia coli"Myeloperoxidase, hydrogen peroxide, chloride antimicrobial system: Nitrogen-chlorine derivatives of bacterial components in bactericidal action against . Infect. Immun. 23 (2): 522–531.  
  5. ^ a b c d Albrich, J. M., C. A. McCarthy, and J. K. Hurst (1981). "Biological reactivity of hypochlorous acid: Implications for microbicidal mechanisms of leukocyte myeloperoxidase". Proc. Natl. Acad. Sci. 78 (1): 210–214.  
  6. ^ Disinfection of Facility H2O.
  7. ^ Water Works: Hyatt's New Disinfectant/Cleaner Comes from the Tap, Bloomberg Businessweek.
  8. ^ a b c d e Fair, G. M., J. Corris, S. L. Chang, I. Weil, and R. P. Burden (1948). "The behavior of chlorine as a water disinfectant". J. Am. Water Works Assoc. 40: 1051–1061. 
  9. ^ a b Inorganic chemistry, Egon Wiberg, Nils Wiberg, Arnold Frederick Holleman , "Hypochlorous acid" p.442 , section 4.3.1
  10. ^ a b c Dennis, W. H., Jr, V. P. Olivieri, and C. W. Krusé (1979). "The reaction of nucleotides with aqueous hypochlorous acid". Water Res 13 (4): 357–362.  
  11. ^ Jacangelo, J. G., and V. P. Olivieri. 1984. Aspects of the mode of action of monochloramine. In R. L. Jolley, R. J. Bull, W. P. Davis, S. Katz, M. H. Roberts, Jr., and V. A. Jacobs (ed.), Water Chlorination, vol. 5. Lewis Publishers, Inc., Williamsburg.
  12. ^ a b c d e Prütz, WA (1998). "Interactions of hypochlorous acid with pyrimidine nucleotides, and secondary reactions of chlorinated pyrimidines with GSH, NADH, and other substrates.". Archives of biochemistry and biophysics 349 (1): 183–91.  
  13. ^ a b c Arnhold, J; Panasenko, OM; Schiller, J; Vladimirov, YuA; Arnold, K (1995). "The action of hypochlorous acid on phosphatidylcholine liposomes in dependence on the content of double bonds. Stoichiometry and NMR analysis.". Chemistry and physics of lipids 78 (1): 55–64.  
  14. ^ a b c Carr, AC; Van Den Berg, JJ; Winterbourn, CC (1996). "Chlorination of cholesterol in cell membranes by hypochlorous acid". Archives of biochemistry and biophysics 332 (1): 63–9.  
  15. ^ a b Carr, AC; Vissers, MC; Domigan, NM; Winterbourn, CC (1997). "Modification of red cell membrane lipids by hypochlorous acid and haemolysis by preformed lipid chlorohydrins". Redox report : communications in free radical research 3 (5–6): 263–71.  
  16. ^ a b c Hazell, L. J., J. V. D. Berg, and R. Stocker (1994). "Oxidation of low density lipoprotein by hypochlorite causes aggregation that is mediated by modification of lysine residues rather than lipid oxidation". Biochem. J. 302: 297–304.  
  17. ^ a b c Hazen, SL; Hsu, FF; Duffin, K; Heinecke, JW (1996). "Molecular chlorine generated by the myeloperoxidase-hydrogen peroxide-chloride system of phagocytes converts low density lipoprotein cholesterol into a family of chlorinated sterols". The Journal of Biological Chemistry 271 (38): 23080–8.  
  18. ^ Vissers, MC; Carr, AC; Chapman, AL (1998). "Comparison of human red cell lysis by hypochlorous and hypobromous acids: insights into the mechanism of lysis". The Biochemical journal 330 (1): 131–8.  
  19. ^ a b Vissers, MC; Stern, A; Kuypers, F; Van Den Berg, J; Winterbourn, CC (1994). "Membrane changes associated with lysis of red blood cells by hypochlorous acid". Free radical biology & medicine 16 (6): 703–12.  
  20. ^ Winterbourn, CC; Van Den Berg, JJ; Roitman, E; Kuypers, FA (1992). "Chlorohydrin formation from unsaturated fatty acids reacted with hypochlorous acid". Archives of biochemistry and biophysics 296 (2): 547–55.  
  21. ^ a b c d Barrette Jr, WC; Hannum, DM; Wheeler, WD; Hurst, JK (1989). "General mechanism for the bacterial toxicity of hypochlorous acid: abolition of ATP production". Biochemistry 28 (23): 9172–8.  
  22. ^ a b c Jacangelo, J; Olivieri, V; Kawata, K (1987). "Oxidation of sulfhydryl groups by monochloramine". Water Research 21 (11): 1339.  
  23. ^ a b c d e Knox, WE; Stumpf, PK; Green, DE; Auerbach, VH (1948). "The Inhibition of Sulfhydryl Enzymes as the Basis of the Bactericidal Action of Chlorine". Journal of bacteriology 55 (4): 451–8.  
  24. ^ Vissers, MC; Winterbourn, CC (1991). "Oxidative damage to fibronectin. I. The effects of the neutrophil myeloperoxidase system and HOCl". Archives of biochemistry and biophysics 285 (1): 53–9.  
  25. ^ a b c Winterbourn, CC (1985). "Comparative reactivities of various biological compounds with myeloperoxidase-hydrogen peroxide-chloride, and similarity of the oxidant to hypochlorite". Biochimica et Biophysica Acta 840 (2): 204–10.  
  26. ^ a b Pereira, WE; Hoyano, Y; Summons, RE; Bacon, VA; Duffield, AM (1973). "Chlorination studies. II. The reaction of aqueous hypochlorous acid with alpha-amino acids and dipeptides". Biochimica et Biophysica Acta 313 (1): 170–80.  
  27. ^ Dychdala, G. R. 1991. Chlorine and chlorine compounds, pp. 131–151. In S. S. Block (ed.), Disinfection, Sterilization and Preservation. Lea & Febiger, Philadelphia. ISBN 0-683-30740-1
  28. ^ a b c McKenna, SM; Davies, KJ (1988). "The inhibition of bacterial growth by hypochlorous acid. Possible role in the bactericidal activity of phagocytes". The Biochemical journal 254 (3): 685–92.  
  29. ^ Hazen, SL; D'avignon, A; Anderson, MM; Hsu, FF; Heinecke, JW (1998). "Human neutrophils employ the myeloperoxidase-hydrogen peroxide-chloride system to oxidize alpha-amino acids to a family of reactive aldehydes. Mechanistic studies identifying labile intermediates along the reaction pathway". The Journal of Biological Chemistry 273 (9): 4997–5005.  
  30. ^ a b c d e Prütz, WA (1996). "Hypochlorous acid interactions with thiols, nucleotides, DNA, and other biological substrates". Archives of biochemistry and biophysics 332 (1): 110–20.  
  31. ^ Rakita, RM; Michel, BR; Rosen, H (1990). "Differential inactivation of Escherichia coli membrane dehydrogenases by a myeloperoxidase-mediated antimicrobial system". Biochemistry 29 (4): 1075–80.  
  32. ^ a b Rakita, RM; Michel, BR; Rosen, H (1989). "Myeloperoxidase-mediated inhibition of microbial respiration: damage to Escherichia coli ubiquinol oxidase". Biochemistry 28 (7): 3031–6.  
  33. ^ Rosen, H., and S. J. Klebanoff (1985). "Oxidation of microbial iron-sulfur centers by the myeloperoxidase-H2O2-halide antimicrobial system". Infect. Immun. 47 (3): 613–618.  
  34. ^ a b c Rosen, H., R. M. Rakita, A. M. Waltersdorph, and S. J. Klebanoff (1987). "Myeloperoxidase-mediated damage to the succinate oxidase system of Escherichia coli". J. Biol. Chem. 242: 15004–15010. 
  35. ^ Chesney, JA; Eaton, JW; Mahoney Jr, JR (1996). "Bacterial glutathione: a sacrificial defense against chlorine compounds". Journal of bacteriology 178 (7): 2131–5.  
  36. ^ a b c Morris, J. C. (1966). "The acid ionization constant of HClO from 5 to 35 °".  
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  45. ^ Winter, J.; Ilbert, M.; Graf, P.C.F.; Özcelik, D.; Jakob, U. (2008). "Bleach Activates a Redox-Regulated Chaperone by Oxidative Protein Unfolding". Cell 135 (4): 691–701.  
  46. ^ Hospital Infection Research Laboratory. Queen Elizabeth Hospital, Birmingham. July 2010
  47. ^ Wang L et al. Hypochlorous acid as a potential wound care agent. Part I Stabilized hypochlorous acid: a component of the inorganic armamentarium of innate immunity. J Burns and Wounds 2007;April:65-79.
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  49. ^ Selkon JB et al 2006. Evaluation of hypochlorous acid washes in the treatment of venous leg ulcers. J Wound Care 2006: 15:33-37.

External links

  • National Pollutant Inventory – Chlorine
  • Reuters – Mystery solved: How bleach kills germs
  • Royal Society of Chemistry-'The Mole' Magazine, MARCH 2014 issue
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