World Library  
Flag as Inappropriate
Email this Article

Laboratory glassware

Article Id: WHEBN0000043958
Reproduction Date:

Title: Laboratory glassware  
Author: World Heritage Encyclopedia
Language: English
Subject: Condenser (laboratory), Conical measure, Dropping funnel, Gas syringe, Volumetric flask
Collection: Laboratory Glassware
Publisher: World Heritage Encyclopedia

Laboratory glassware

Late 17th-century laboratory glassware in the painting by Cornelis de Man (National Museum in Warsaw).

Laboratory glassware refers to a variety of equipment, traditionally made of glass, used for scientific experiments and other work in science, especially in chemistry and biology laboratories.


  • Applications 1
    • Examples 1.1
  • Production 2
  • Features and accessories 3
    • Stoppers 3.1
    • Coatings 3.2
    • Connectors 3.3
    • Glassware valves 3.4
      • Stopcock valve 3.4.1
      • Threaded plug valve 3.4.2
      • Glass valves 3.4.3
    • Fritted glass 3.5
  • Gallery 4
  • Functions 5
    • Heating reagents or samples 5.1
    • Negative pressure and vacuums 5.2
    • Hermetic sealing 5.3
    • Cleaning 5.4
    • Disposal 5.5
  • References 6


Brown glass jars with some clear lab glassware in the background

Glass use in laboratory applications is not as commonplace as it once was because of cheaper, less breakable, plasticware; however, certain applications still require glassware because glass is relatively inert, transparent, heat-resistant, and easy to customize. The type of glass used is dependent on the application. Borosilicate glass, which is commonly used in reagent bottles, can withstand thermal stress. Quartz glass, which is common in cuvettes, can withstand high temperatures and is transparent in certain parts of the electromagnetic spectrum. Darkened brown or amber (actinic) glass, which is common in dark storage bottles, can block ultraviolet and infrared radiation. Heavy-wall glass, which is common in glass pressure reactors, can withstand pressurized applications.


Beakers are commonly used for holding reagents.

There are many different kinds of laboratory glassware items.[1] These include:

  • Beakers are simple cylindrical shaped containers used to hold reagents or samples.
  • Flasks are narrow-necked glass containers, typically conical or spherical, used in a laboratory to hold reagents or samples.
  • Bottles are containers with narrow openings generally used to store reagents or samples. Small bottles are called vials.
  • Jars are cylindrical containers with wide openings that may be sealed. Bell jars are used to contain vacuums.
  • Watch glasses are shallow glass dishes used as an evaporating surface or to cover a beaker.
  • Graduated cylinders are cylindrical containers used for volumetric measurements.
  • Stirring rods are used to mix chemicals.
  • Burettes are used to disperse precise amounts of liquid reagents.
  • Condensers are used to cool hot liquids or vapors.
  • Funnels are used to get materials through a narrow opening.
  • Desiccators of glass construction are used to dry materials or keep material dry.
  • Glass tubes are cylindrical pieces of glassware used to hold or transport materials.
  • Glass retorts are used for distillation.
  • Glass pipettes are used to transport precise quantities of fluids.
  • Glass petri dishes are used to culture living cells.
  • Drying pistols are used to free samples from traces of water, or other impurities.
  • Glass evaporating dishes are used to evaporate materials.
  • Microscope slides are thin strips used to hold items under a microscope.


Most laboratory glassware is currently mass-produced, but large laboratories may employ a glass blower to construct specialized pieces. This construction forms a specialized field of glassblowing requiring precise control of shape and dimension. In addition to repairing expensive or difficult-to-replace glassware, scientific glassblowing commonly involves fusing together various glass parts—such as glass joints and tubing, stopcocks, transition pieces, and/or other glassware or parts of them to form items of glassware, such as vacuum manifolds, special reaction flasks, etc.

Various types of joints and stopcocks are available separately and come fused with a length of glass tubing, which a glassblower may use to fuse to another piece of glassware.

Features and accessories

When in use, laboratory glassware is often held in place with clamps made for that purpose, which are likewise attached and held in place by stands or racks. This article covers aspects of laboratory glassware which may be common to several kinds of glassware and may briefly describe a few glassware items not covered in other articles. Describing glassware can be complicated since manufacturers provide conflicting names for glassware. For example, what ChemGlass calls a glass stopcock, Kontes calls a glass plug.


Stoppers are used to seal certain types of glassware. These may be made out of glass as well as other materials including rubber or cork.


Glassware may be coated to reduce the occurrence of breakage.


An Erlenmeyer and a filtering flask. Note the barbed sidearm on the filtering flask
A glass adapter with a hose barb on the left and a ground glass connector on the right
  • Ground glass joints composed of all glass quickly and easily fit leak-tight apparatus together.
  • Glass connectors or adapters are hollow glassware components with ground glass joints open at one or both ends.
  • Hose barbs are cylindrical tubes with barbs used to attach hoses and flexible tubing to glassware.
  • Keck clips and other clamping methods can be used to hold glassware together.
  • Glass tubes, T-connectors, and Y-connectors may be used as interconnecting components.
  • Rubber bungs or stoppers may hold glassware together.

Glassware valves

Valves are used to redirect flows through pipes. Two types of valves used in laboratory glassware are the stopcock valve and the threaded plug valve. These and other terms used below are defined in detail since they are bound to conflict with different sources.

Stopcock valve

Stopcocks are a smooth tampered plug or rotor with a handle, which fits into a corresponding ground glass female joint. Stopcocks are often parts of laboratory glassware such as burettes, separatory funnels, Schlenk flasks, and columns used for column chromatography.

Threaded plug valve

Threaded plug valves are used significantly in air-sensitive chemistry as well as when a vessel must be closed completely as in the case of Schlenk bombs. The construction of a threaded plug valve involves a plug with a threaded cap which are made so that they fit with the threading on a corresponding piece of female glass. Screwing the plug in part-way first engages one or more O-rings, made of rubber or plastic, near the plug's base, which seals the female joint off from the outer atmosphere. Screwing the plug valve all the way in engages the plug's tip with a beveled constriction in the glass, which provides a second seal. This seal separates the region beyond the bevel and the O-rings already mentioned.

With solid plugs, a tube or area exists above and below the bevel and turning the plug controls access. In a number of cases it is convenient to fully remove a plug which can give access to the region beyond the bevel. Plugs are generally made of an inert plastic such as PTFE and are attached to a threaded sleeve in such a way that the sleeve can be turned without spinning the plug. The contact with the bevel is made by an O-ring fitted to the tip of the plug or by the plug itself. There are a few examples where the plug in made of glass. In the case of glass plugs, the joint contact is always a rubber O-ring but they are still prone to shattering.

Not all plugs are solid. Some plugs are bored with a T-junction. In these systems the plug extends beyond the threaded sleeve and is designed to form an airtight fitting with glass tubing or hosing. The shaft of the plug is bored from beyond the threaded sleeve to a T-junction just before the bevel plug contact. When the plug is fully sealed, the region beyond the bevel is separated from the plug shaft as well as the bore which leads out of its shaft. When the plug bevel contact is released, the two regions are exposed to each other. These valves have also been used as a grease-free alternative to straight bored stopcocks common to Schlenk flasks. The high symmetry and concise design of these valves has also made them popular for capping NMR tubes. Such NMR tubes can be heated without the loss of solvent thanks to the valve's gas-tight seal. NMR tubes with T-bore plugs are widely known as J. Young NMR tubes, named after the brand name of valves most commonly used for this purpose. Images of J. Young NMR tubes and a J. Young NMR tube adapter are in the gallery.

Glass valves

An all-glass check valve

Valves made entirely of glass may be used to restrict fluid flows.

Fritted glass

Fritted glass is finely porous glass through which gas or liquid may pass. Applications in laboratory glassware include use in fritted glass filter items, scrubbers, or spargers. Other laboratory applications of fritted glass include packing in chromatography columns and resin beds for special chemical synthesis.



Glass has a wide variety of functions which include volumetric measuring, holding or storing vacuum, and pressure, like pressure reactor.

Laboratory glassware may be part of a sophisticated apparatus, as is the case with certain types of condensers, and it may be used in conjunction with other laboratory equipment such as ring stands, burette clamps, and bunsen burners.

Heating reagents or samples

Heating or cooling of most glassware must be done carefully because thermal expansion in one portion of the glass but not an adjacent portion may put too much mechanical stress on the surface and cause it to fracture. Fracturing is a concern when students new to laboratory become impatient and heat glassware, especially the larger pieces, too fast. Heating of glassware should be slowed using an insulating material, such as metal foil or wool, or specialized equipment such as heated baths, heating mantles or laboratory grade hot plates to avoid fracturing.

Hot glass also looks exactly like cold glass, so care must be taken to avoid grabbing hot glassware.[2]

Negative pressure and vacuums

A bell jar operating at a negative pressure

An absolute vacuum produces a pressure difference of one atmosphere, approximately 14 psi, over the surface of the glass. The energy contained within an implosion is defined by the pressure difference and the volume evacuated. Flask volumes can change by orders of magnitude between experiments. Whenever working with liter sized or larger flasks, chemists should consider using a safety screen or the sash of a flow hood to protect them from shards of glass, should an implosion occur. Glassware can also be wrapped with spirals of tape to catch shards, or wrapped with webbed mesh more commonly seen on scuba cylinders.

Glass under vacuum becomes more sensitive to chips and scratches in its surface, as these form strain accumulation points, so older glass is best avoided if possible. Impacts to the glass and thermally induced stresses are also concerns under vacuum. Round bottom flasks more effectively spread the stress across their surfaces, and are therefore safer when working under vacuum.

When connecting glassware, it is often tempting to use Keck clips on every joint, but this can be dangerous if the system is sealed or the exhaust is in any way restricted; e.g. by wash flasks or drying media. Many reactions and forms of operation can produce sudden, unexpected surges of pressure inside the glass. If the system is sealed or restricted, this can blow the glass apart. It is safer to only clip the joints that need holding together to stop them falling apart and to purposefully leave one or more unclipped; preferably those that are connected to lightweight, small objects like stoppers, thermometers or wash heads, that are pointing vertically upwards and not connected to other items of glassware. By doing so, any significant surge of pressure will cause these specifically chosen tapers to open and vent. This may seem counterintuitive, but it is safer and easier to deal with a controlled escape as opposed to the entire volume being uncontrollably released in an explosion.

Hermetic sealing

Hermetic sealing of laboratory glassware involves using a thin layer of Polytetrafluoroethylene (PTFE) material or grease to get an airtight seal. The seal may be applied to the ground-glass surfaces to be connected, and the inner joint is inserted into the outer joint such that the ground-glass surfaces of each are next to each other to make the connection. This helps provide a good seal and prevents the joint from seizing, allowing the parts to be disassembled easily.

Sealing allows chemists to easily see when a taper is leaking, as bubbles can usually be seen flowing through the taper. PTFE tape, bands, and fluoroether-based grease or oils, but not silicone-based, all emit hydrogen fluoride fumes as they approach and exceed their working temperature limits, which can occur when using a hotplate, mantle, oil bath or flame. Upon contact with moisture, including tissue, hydrogen fluoride immediately converts to hydrofluoric acid, which is highly corrosive and toxic, and requires immediate medical attention upon exposure.


There are many different methods of cleaning laboratory glassware. Most of the time, these methods[3][4] are tried in this order:

  • A detergent solution may be used to soak glassware. This removes grease and loosens most contamination.
  • Scrubbing with a brush or scouring pad is a mechanical means of removing gross contamination and large particles.
  • Sonicating the glassware in a hot detergent solution is an alternative to both a detergent solution and scrubbing.
  • Solvents, such as mild acids, known to dissolve a specific contamination may be used to remove trace quantities.
  • Acetone may be used for a final rinse of sensitive or urgently needed glassware as the solvent is miscible with water, and helps dilute and wash away remaining water from the glassware.
  • Glassware is often dried by suspending it upside down to drip dry on racks; these can include a hot air fan to blow the internals dry. Another alternative is to place the glassware under vacuum, lower the boiling points of the remaining volatiles.
  • For special type of precipitates, aqua regia (concentrated HCL: HNO3 at 3:1 ratio) is used.


Typically broken glassware must be disposed of separately because shattered glass may be sharp.


  1. ^ Dr. Anne M. Helmenstine (2013-03-11). "Chemistry Laboratory Glassware Gallery".  
  2. ^ "Glassware Safety". Univ Nevada Reno. Retrieved 2013-04-11. 
  3. ^ "Suggestions for Cleaning Laboratory Glassware".  
  4. ^ J. M. McCormick (2006-06-30). "The Grasshopper's Guide to Cleaning Glassware".  
This article was sourced from Creative Commons Attribution-ShareAlike License; additional terms may apply. World Heritage Encyclopedia content is assembled from numerous content providers, Open Access Publishing, and in compliance with The Fair Access to Science and Technology Research Act (FASTR), Wikimedia Foundation, Inc., Public Library of Science, The Encyclopedia of Life, Open Book Publishers (OBP), PubMed, U.S. National Library of Medicine, National Center for Biotechnology Information, U.S. National Library of Medicine, National Institutes of Health (NIH), U.S. Department of Health & Human Services, and, which sources content from all federal, state, local, tribal, and territorial government publication portals (.gov, .mil, .edu). Funding for and content contributors is made possible from the U.S. Congress, E-Government Act of 2002.
Crowd sourced content that is contributed to World Heritage Encyclopedia is peer reviewed and edited by our editorial staff to ensure quality scholarly research articles.
By using this site, you agree to the Terms of Use and Privacy Policy. World Heritage Encyclopedia™ is a registered trademark of the World Public Library Association, a non-profit organization.

Copyright © World Library Foundation. All rights reserved. eBooks from World eBook Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.